منابع مشابه
Insights into the Fold Organization of TIM Barrel from Interaction Energy Based Structure Networks
There are many well-known examples of proteins with low sequence similarity, adopting the same structural fold. This aspect of sequence-structure relationship has been extensively studied both experimentally and theoretically, however with limited success. Most of the studies consider remote homology or "sequence conservation" as the basis for their understanding. Recently "interaction energy" ...
متن کاملDe novo design of a four-fold symmetric TIM-barrel protein with atomic-level accuracy.
Despite efforts for over 25 years, de novo protein design has not succeeded in achieving the TIM-barrel fold. Here we describe the computational design of four-fold symmetrical (β/α)8 barrels guided by geometrical and chemical principles. Experimental characterization of 33 designs revealed the importance of side chain-backbone hydrogen bonds for defining the strand register between repeat unit...
متن کاملReverse engineering the (bya)8 barrel fold
The (bya)8 barrel is the most commonly occurring fold among protein catalysts. To lay a groundwork for engineering novel barrel proteins, we investigated the amino acid sequence restrictions at 182 structural positions of the prototypical (bya)8 barrel enzyme triosephosphate isomerase. Using combinatorial mutagenesis and functional selection, we find that turn sequences, a-helix capping and sto...
متن کاملPhotoinduced, family-specific, site-selective cleavage of TIM-barrel proteins.
Nonenzymatic, chemical methods for the controlled cleavage of proteins at predictable sites in a site-specific manner are rare and of strong potential utility in clean, post-translational manipulation of protein structure for use in, for example, proteomics, sequencing, and tagged-protein production. Unprecedented photochemical, site-selective cleavage of a His-Trp (HW) motif in the GH1 family ...
متن کاملEvolutionary markers in the (b/a)8-barrel fold
Enzymes with the (b/a)8-barrel fold are involved in the catalysis of a wide variety of biochemical reactions. The active sites of these enzymes are located on the C-terminal face of the central b-barrel. Conserved amino acid sequence, as well as secondary, tertiary and quaternary structure patterns are providing a rich body of data to support the premise of a common ancestry of many members of ...
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ژورنال
عنوان ژورنال: WikiJournal of Science
سال: 2020
ISSN: 2470-6345
DOI: 10.15347/wjs/2020.004